mcl-1 overexpression plasmid Search Results


mcl1 overexpression plasmid pcdna- mcl1  (Genechem)
90
Genechem mcl1 overexpression plasmid pcdna- mcl1
FAM83D is highly expressed in tumor tissues and is associated with patient prognosis. (A) The expression of FAM83D was higher in TCGA-LIHC tumor tissues than non-tumor tissues. (B) Data in GEO GSE25097 and GSE76427 show that the level of FAM83D is higher in HCC tissues than non-tumor tissues. (C) The expression of FAM83D in TCGA-LIHC tissues based on tumor grade. (D) The expression of FAM83D in TCGA-LIHC tissues based on individual cancer stages. (E) Patients with high levels of FAM83D had significantly lower overall survival than patients with low levels of FAM83D . (F) FAM83D protein expression in 5 HCC cell lines. (G) The effect of siRNA knockdown on FAM83D in SNU449 cells was verified by Western blotting. (H) The effect of siRNA knockdown on FAM83D in HUH7 cells was verified by Western blotting. (I) The effect of <t>pcDNA</t> overexpress on FAM83D in BEL7402 cells was verified by Western blotting. *P<0.05; **P<0.01. TPM, transcripts per million; TCGA-LIHC, The Cancer Genome Atlas-Liver Hepatocellular Carcinoma; GEO, Gene Expression Omnibus; HCC, hepatocellular carcinoma.
Mcl1 Overexpression Plasmid Pcdna Mcl1, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcl1 overexpression plasmid pcdna- mcl1/product/Genechem
Average 90 stars, based on 1 article reviews
mcl1 overexpression plasmid pcdna- mcl1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mcl1 (nm_021960) human untagged clone  (OriGene)
89
OriGene mcl1 (nm_021960) human untagged clone
FAM83D is highly expressed in tumor tissues and is associated with patient prognosis. (A) The expression of FAM83D was higher in TCGA-LIHC tumor tissues than non-tumor tissues. (B) Data in GEO GSE25097 and GSE76427 show that the level of FAM83D is higher in HCC tissues than non-tumor tissues. (C) The expression of FAM83D in TCGA-LIHC tissues based on tumor grade. (D) The expression of FAM83D in TCGA-LIHC tissues based on individual cancer stages. (E) Patients with high levels of FAM83D had significantly lower overall survival than patients with low levels of FAM83D . (F) FAM83D protein expression in 5 HCC cell lines. (G) The effect of siRNA knockdown on FAM83D in SNU449 cells was verified by Western blotting. (H) The effect of siRNA knockdown on FAM83D in HUH7 cells was verified by Western blotting. (I) The effect of <t>pcDNA</t> overexpress on FAM83D in BEL7402 cells was verified by Western blotting. *P<0.05; **P<0.01. TPM, transcripts per million; TCGA-LIHC, The Cancer Genome Atlas-Liver Hepatocellular Carcinoma; GEO, Gene Expression Omnibus; HCC, hepatocellular carcinoma.
Mcl1 (Nm 021960) Human Untagged Clone, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcl1 (nm_021960) human untagged clone/product/OriGene
Average 89 stars, based on 1 article reviews
mcl1 (nm_021960) human untagged clone - by Bioz Stars, 2026-03
89/100 stars
  Buy from Supplier

Image Search Results


FAM83D is highly expressed in tumor tissues and is associated with patient prognosis. (A) The expression of FAM83D was higher in TCGA-LIHC tumor tissues than non-tumor tissues. (B) Data in GEO GSE25097 and GSE76427 show that the level of FAM83D is higher in HCC tissues than non-tumor tissues. (C) The expression of FAM83D in TCGA-LIHC tissues based on tumor grade. (D) The expression of FAM83D in TCGA-LIHC tissues based on individual cancer stages. (E) Patients with high levels of FAM83D had significantly lower overall survival than patients with low levels of FAM83D . (F) FAM83D protein expression in 5 HCC cell lines. (G) The effect of siRNA knockdown on FAM83D in SNU449 cells was verified by Western blotting. (H) The effect of siRNA knockdown on FAM83D in HUH7 cells was verified by Western blotting. (I) The effect of pcDNA overexpress on FAM83D in BEL7402 cells was verified by Western blotting. *P<0.05; **P<0.01. TPM, transcripts per million; TCGA-LIHC, The Cancer Genome Atlas-Liver Hepatocellular Carcinoma; GEO, Gene Expression Omnibus; HCC, hepatocellular carcinoma.

Journal: Translational Cancer Research

Article Title: FAM83D promotes the proliferation and migration of hepatocellular carcinoma cells by inhibiting the FBXW7/MCL1 pathway

doi: 10.21037/tcr-22-2069

Figure Lengend Snippet: FAM83D is highly expressed in tumor tissues and is associated with patient prognosis. (A) The expression of FAM83D was higher in TCGA-LIHC tumor tissues than non-tumor tissues. (B) Data in GEO GSE25097 and GSE76427 show that the level of FAM83D is higher in HCC tissues than non-tumor tissues. (C) The expression of FAM83D in TCGA-LIHC tissues based on tumor grade. (D) The expression of FAM83D in TCGA-LIHC tissues based on individual cancer stages. (E) Patients with high levels of FAM83D had significantly lower overall survival than patients with low levels of FAM83D . (F) FAM83D protein expression in 5 HCC cell lines. (G) The effect of siRNA knockdown on FAM83D in SNU449 cells was verified by Western blotting. (H) The effect of siRNA knockdown on FAM83D in HUH7 cells was verified by Western blotting. (I) The effect of pcDNA overexpress on FAM83D in BEL7402 cells was verified by Western blotting. *P<0.05; **P<0.01. TPM, transcripts per million; TCGA-LIHC, The Cancer Genome Atlas-Liver Hepatocellular Carcinoma; GEO, Gene Expression Omnibus; HCC, hepatocellular carcinoma.

Article Snippet: FAM83D siRNA (i.e., si FAM83D -1, si FAM83D -2, and si FAM83D -3), FBXW7 siRNA (si FBXW7 ), siRNA negative control (siNC), FAM83D overexpression plasmid pcDNA- FAM83D (pcDNA- FAM83D ), MCL1 overexpression plasmid pcDNA- MCL1 (pcDNA- MCL1 ), and pcDNA negative control (pcDNA-NC) were purchased from Genechem Company (Shanghai, China).

Techniques: Expressing, Knockdown, Western Blot, Gene Expression

FAM83D promotes the proliferation of HCC cells in vitro . (A) MTT assays were used to assess the viability of FAM83D knockdown in HCC cells. (B,C) FAM83D knockdown suppressed the proliferation of SNU449 and HUH7 cells as measured by the EDU analysis (200×). (D) The overexpression of FAM83D promoted the proliferation of BEL7402 cells as measured by the EDU analysis (200×). **P<0.01. HCC, hepatocellular carcinoma; OD, optical density.

Journal: Translational Cancer Research

Article Title: FAM83D promotes the proliferation and migration of hepatocellular carcinoma cells by inhibiting the FBXW7/MCL1 pathway

doi: 10.21037/tcr-22-2069

Figure Lengend Snippet: FAM83D promotes the proliferation of HCC cells in vitro . (A) MTT assays were used to assess the viability of FAM83D knockdown in HCC cells. (B,C) FAM83D knockdown suppressed the proliferation of SNU449 and HUH7 cells as measured by the EDU analysis (200×). (D) The overexpression of FAM83D promoted the proliferation of BEL7402 cells as measured by the EDU analysis (200×). **P<0.01. HCC, hepatocellular carcinoma; OD, optical density.

Article Snippet: FAM83D siRNA (i.e., si FAM83D -1, si FAM83D -2, and si FAM83D -3), FBXW7 siRNA (si FBXW7 ), siRNA negative control (siNC), FAM83D overexpression plasmid pcDNA- FAM83D (pcDNA- FAM83D ), MCL1 overexpression plasmid pcDNA- MCL1 (pcDNA- MCL1 ), and pcDNA negative control (pcDNA-NC) were purchased from Genechem Company (Shanghai, China).

Techniques: In Vitro, Knockdown, Over Expression

FAM83D promotes migration and invasion of HCC cells in vitro . (A) Colony formation experiments of control and FAM83D knockdown and overexpressed HCC cells. Top: representative images; bottom: quantification of colony numbers (crystal violet staining). (B) FAM83D knockdown suppressed SNU449 and HUH7 cell migration ability, and the overexpression of FAM83D promoted BEL7402 cell migration ability as measured by the scratch-healing experiment (40×). (C) Western blot analysis showed that the migration protein level of knocked down FAM83D cells was significantly downregulated, while the migration protein level of over expressed FAM83D cells was significantly upregulated. **P<0.01. HCC, hepatocellular carcinoma.

Journal: Translational Cancer Research

Article Title: FAM83D promotes the proliferation and migration of hepatocellular carcinoma cells by inhibiting the FBXW7/MCL1 pathway

doi: 10.21037/tcr-22-2069

Figure Lengend Snippet: FAM83D promotes migration and invasion of HCC cells in vitro . (A) Colony formation experiments of control and FAM83D knockdown and overexpressed HCC cells. Top: representative images; bottom: quantification of colony numbers (crystal violet staining). (B) FAM83D knockdown suppressed SNU449 and HUH7 cell migration ability, and the overexpression of FAM83D promoted BEL7402 cell migration ability as measured by the scratch-healing experiment (40×). (C) Western blot analysis showed that the migration protein level of knocked down FAM83D cells was significantly downregulated, while the migration protein level of over expressed FAM83D cells was significantly upregulated. **P<0.01. HCC, hepatocellular carcinoma.

Article Snippet: FAM83D siRNA (i.e., si FAM83D -1, si FAM83D -2, and si FAM83D -3), FBXW7 siRNA (si FBXW7 ), siRNA negative control (siNC), FAM83D overexpression plasmid pcDNA- FAM83D (pcDNA- FAM83D ), MCL1 overexpression plasmid pcDNA- MCL1 (pcDNA- MCL1 ), and pcDNA negative control (pcDNA-NC) were purchased from Genechem Company (Shanghai, China).

Techniques: Migration, In Vitro, Control, Knockdown, Staining, Over Expression, Western Blot

FAM83D attenuate the apoptosis of HCC cells in vitro . (A,B) Flow cytometry results showed that FAM83D knockdown promoted cell apoptosis and the apoptotic-related protein level as measured by western blot analysis. (C) Flow cytometry results showed that FAM83D overexpression inhibited cell apoptosis and the apoptotic-related protein level as measured by western blot analysis. **P<0.01. HCC, hepatocellular carcinoma.

Journal: Translational Cancer Research

Article Title: FAM83D promotes the proliferation and migration of hepatocellular carcinoma cells by inhibiting the FBXW7/MCL1 pathway

doi: 10.21037/tcr-22-2069

Figure Lengend Snippet: FAM83D attenuate the apoptosis of HCC cells in vitro . (A,B) Flow cytometry results showed that FAM83D knockdown promoted cell apoptosis and the apoptotic-related protein level as measured by western blot analysis. (C) Flow cytometry results showed that FAM83D overexpression inhibited cell apoptosis and the apoptotic-related protein level as measured by western blot analysis. **P<0.01. HCC, hepatocellular carcinoma.

Article Snippet: FAM83D siRNA (i.e., si FAM83D -1, si FAM83D -2, and si FAM83D -3), FBXW7 siRNA (si FBXW7 ), siRNA negative control (siNC), FAM83D overexpression plasmid pcDNA- FAM83D (pcDNA- FAM83D ), MCL1 overexpression plasmid pcDNA- MCL1 (pcDNA- MCL1 ), and pcDNA negative control (pcDNA-NC) were purchased from Genechem Company (Shanghai, China).

Techniques: In Vitro, Flow Cytometry, Knockdown, Western Blot, Over Expression

FAM83D activates FBXW7 / MCL1 pathway in HCC cells in vitro (HCC cells transfected with FAM83D siRNA; si FAM83D + si FBXW7 ; si FAM83D + pc MCL1 ). (A) The expression levels of FBXW7 and MCL1 proteins in HUH7 and SNU449 cells when FAM83D was knocked down as measured by a western blot analysis. (B) Cell proliferation was measured by MTT assays. (C) Cell migration ability was measured by scratch-healing experiments (40×). **P<0.01; ## P<0.01; HCC, hepatocellular carcinoma; OD, optical density.

Journal: Translational Cancer Research

Article Title: FAM83D promotes the proliferation and migration of hepatocellular carcinoma cells by inhibiting the FBXW7/MCL1 pathway

doi: 10.21037/tcr-22-2069

Figure Lengend Snippet: FAM83D activates FBXW7 / MCL1 pathway in HCC cells in vitro (HCC cells transfected with FAM83D siRNA; si FAM83D + si FBXW7 ; si FAM83D + pc MCL1 ). (A) The expression levels of FBXW7 and MCL1 proteins in HUH7 and SNU449 cells when FAM83D was knocked down as measured by a western blot analysis. (B) Cell proliferation was measured by MTT assays. (C) Cell migration ability was measured by scratch-healing experiments (40×). **P<0.01; ## P<0.01; HCC, hepatocellular carcinoma; OD, optical density.

Article Snippet: FAM83D siRNA (i.e., si FAM83D -1, si FAM83D -2, and si FAM83D -3), FBXW7 siRNA (si FBXW7 ), siRNA negative control (siNC), FAM83D overexpression plasmid pcDNA- FAM83D (pcDNA- FAM83D ), MCL1 overexpression plasmid pcDNA- MCL1 (pcDNA- MCL1 ), and pcDNA negative control (pcDNA-NC) were purchased from Genechem Company (Shanghai, China).

Techniques: In Vitro, Transfection, Expressing, Western Blot, Migration

FAM83D activates FBXW7 / MCL1 pathway in HCC cells in vitro (HCC cells transfected with FAM83D siRNA; si FAM83D + si FBXW7 ; si FAM83D + pc MCL1 ). (A) The migration protein level was measured by western blot analysis. (B) Cell apoptosis was measured by flow cytometry. (C) The apoptotic-related protein level was measured by western blot analysis. **P<0.01; ## P<0.01.

Journal: Translational Cancer Research

Article Title: FAM83D promotes the proliferation and migration of hepatocellular carcinoma cells by inhibiting the FBXW7/MCL1 pathway

doi: 10.21037/tcr-22-2069

Figure Lengend Snippet: FAM83D activates FBXW7 / MCL1 pathway in HCC cells in vitro (HCC cells transfected with FAM83D siRNA; si FAM83D + si FBXW7 ; si FAM83D + pc MCL1 ). (A) The migration protein level was measured by western blot analysis. (B) Cell apoptosis was measured by flow cytometry. (C) The apoptotic-related protein level was measured by western blot analysis. **P<0.01; ## P<0.01.

Article Snippet: FAM83D siRNA (i.e., si FAM83D -1, si FAM83D -2, and si FAM83D -3), FBXW7 siRNA (si FBXW7 ), siRNA negative control (siNC), FAM83D overexpression plasmid pcDNA- FAM83D (pcDNA- FAM83D ), MCL1 overexpression plasmid pcDNA- MCL1 (pcDNA- MCL1 ), and pcDNA negative control (pcDNA-NC) were purchased from Genechem Company (Shanghai, China).

Techniques: In Vitro, Transfection, Migration, Western Blot, Flow Cytometry